Journal: bioRxiv
Article Title: A genetically engineered vertebrate animal model of NAA15 -related heart disease uncovers myocardial growth, contractility, and mitochondrial defects
doi: 10.1101/2025.08.04.668548
Figure Lengend Snippet: (A) Brightfield ventral images of 4dpf control-sibling (CTRL) and double knockout (DKO) animals stained with Alcian Blue to visualize cartilage. (B) Confocal projections of ventral head muscles in 3 dpf CTRL (n=5) and DKO (n=6) animals stained for striated muscle (MF20 antibody). For both (A) and (B), similar staining patterns were observed across all animals in each experimental group. Abbr: V, ventricle; A, atrium; OFT, outflow tract; ventral PA1 (mandibular) muscle: ima, intermandibular anterior; middle PAI muscles: imp, intermandibular posterior and am, adductor mandibulae; ventral PAII (hyoid) muscle: ih, interhyal; middle PA2 muscle: hh, hyohyal; VA, ventral aorta; PAI cartilage: m, meckel’s and pq, palatoquadrate; PAII cartilage: ch, ceratohyal; PAIII-VII cartilage: cb, ceratobranchial cartilage.
Article Snippet: The following antibodies were used: anti-GFP (1:1000, chicken anti-GFP, Aves Labs), MF20 anti-sarcomeric myosin heavy chain [1:50, Developmental Studies Hybridoma Bank (DSHB)], anti-Elastin 2b (1:1000), ZN-8 anti-Alcama (1:50, DHSB), CH1 anti-sarcomeric tropomyosin (1:200, DHSB), and anti-dsRed (1:500, Clontech, Cat# 632496).
Techniques: Control, Double Knockout, Staining, Muscles